International basic research publications in which ciPTEC have been used:


  1. Jansen K et al., Fabrication of Kidney Proximal Tubule Grafts Using Biofunctionalized Electrospun Polymer Scaffolds. Macromol Biosci. 2018 doi: 10.1002/mabi.201800412
  2. Di Mise A et al. Activation of the Calcium-Sensing Receptor Corrects the Impaired Mitochondrial Energy Status Observed in Renal Polycystin-1 Knockdown Cells Modeling Autosomal Dominant Polycystic Kidney Disease. Front Mol Biosci. 2018 doi: 10.3389/fmolb.2018.00077
  3. Sjögren AK et al. A novel multi-parametric high content screening assay in ciPTEC-OAT1 to predict drug-induced nephrotoxicity during drug discovery. Arch Toxicol. 2018 doi: 10.1007/s00204-018-2284-y
  4. Vormann MK et al. Nephrotoxicity and Kidney Transport Assessment on 3D Perfused Proximal Tubules. AAPS J. 2018 doi: 10.1208/s12248-018-0248-z
  5. Vriend J et al. Screening of Drug-Transporter Interactions in a 3D Microfluidic Renal Proximal Tubule on a Chip. AAPS J. 2018 doi: 10.1208/s12248-018-0247-0
  6. Raaij, van SEG et al. Inhibition of Nrf2 alters cell stress induced by chronic iron exposure in human proximal tubular epithelial cells. Toxicol Lett. 2018 doi: 10.1016/j.toxlet.2018.06.1218
  7. Fedecostante M et al. Recellularized native kidney scaffolds as a novel tool in nephrotoxicity screening. Drug Metab Dispos. 2018 doi: 10.1124/dmd.118.080721
  8. Suter-Dick L et al. Combining extracellular miRNA determination with microfluidic 3D-cell cultures for the assessment of nephrotoxicity: A proof-of-concept study. AAPS J 2018 doi: 10.1208/s12248-018-0245-2
  9. Chevtchik NV et al. A bioartificial kidney device with polarized secretion of immune modulators. J Tissue Eng Regen Med. 2018 doi: 10.1002/term.2694
  10. Di Mise A et al. Activation of Calcium-Sensing Receptor increases intracellular calcium and decreases cAMP and mTOR in PKD1 deficient cells. Sci Rep. 2018 doi: 10.1038/s41598-018-23732-5
  11. Bellomo F et al. Impact of atypical mitochondrial cyclic-AMP level in nephropathic cystinosis. Cell Mol Life Sci. 2018 doi: 10.1007/s00018-018-2800-5
  12. Jamalpoor A et al. Quantification of cystine in human renal proximal tubule cells using liquid chromatography-tandem mass spectrometry. Biomed Chromatogr. 2018 doi: 10.1002/bmc.4238
  13. Nieskens TT et al. Expression of organic anion transporter 1 or 3 in human kidney proximal tubule cells reduces cisplatin sensitivity. Drug Metab Dispos. 2018 doi: 10.1124/dmd.117.079384
  14. Mihajlovic M et al. Role of Vitamin D in Maintaining Renal Epithelial Barrier Function in Uremic Conditions. Int J Mol Sci. 2017 doi: 10.3390/ijms18122531.
  15. Weber HM et al. Heparin-based hydrogels induce human renal tubulogenesis in vitro. Acta Biomater. 2017 doi: 10.1016/j.actbio.2017.05.035
  16. Schirris TJJet al. Mild intracellular acidification by dexamethasone attenuates mitochondrial dysfunction in a human inflammatory proximal tubule epithelial cell model. Sci Rep. 2017 doi: 10.1038/s41598-017-10483-y
  17. Mihajlovic M et al. Allostimulatory capacity of conditionally immortalized proximal tubule cell lines for bioartificial kidney application. Scientific Reports. 2017 doi: 10.1038/s41598-017-07582-1
  18. Reda A et al. A Human Proximal Tubular Epithelial Cell Model to Explore a Knowledge Gap on Neonatal Drug Disposition. Curr Pharm Des. 2017 doi: 10.2174/1381612823666171009143146.
  19. Caetano-Pinto P. et al. Cetuximab prevents methotrexate-induced cytotoxicity in vitrothrough epidermal growth factor dependent regulation of renal drug transporters. Mol Pharmaceutics. 2017 doi: 10.1021/acs.molpharmaceut.7b00308
  20. Peters E et al. Effects of a human recombinant alkaline phosphatase during impaired mitochondrial function in human renal proximal tubule epithelial cells. Eur J Pharmacol. 2017 doi: 10.1016/j.ejphar.2016.12.034.
  21. Ivanova EA et al. Ca(2+) signalling in human proximal tubular epithelial cells deficient for cystinosin. Cell Calcium. 2016 doi: 10.1016/j.ceca.2016.07.001.
  22. Ivanova EA et al. Altered mTOR signalling in nephropathic cystinosis. J Inherit Metab Dis. 2016 doi: 10.1007/s10545-016-9919-z
  23. Hulshof F et al. New insights into the effects of biomaterial chemistry and topography on the morphology of kidney epithelial cells. J Tissue Eng Regen Med. 2016 doi: 10.1002/term.2387
  24. Huang J et al. Fluorescently Labeled Cyclodextrin Derivatives as Exogenous Markers for Real-Time Transcutaneous Measurement of Renal Function. Bioconjug Chem. 2016; doi: 10.1021/acs.bioconjchem.6b00452
  25. Chevtchik et al. Upscaling of a living membrane for bioartificial kidney device. Eur J Pharmacol. 2016 doi: 10.1016/j.ejphar.2016.07.009
  26. Jansen J et al. Bioengineered kidney tubules efficiently excrete uremic toxins. Scientific Reports. Sci Rep. 2016 doi: 10.1038/srep26715
  27. Arcolino FO et al. Urine of Preterm Neonates as a Novel Source of Kidney Progenitor Cells. J Am Soc Nephrol. 2016 Ivanova EA et al. Endo-lysosomal dysfunction in human proximal tubular epithelial cells deficient for lysosomal cystine transporter cystinosin. PLoS One. 2015 doi: 10.1371/journal.pone.0120998
  28. Caetano-Pinto P. et al. Fluorescence based transport assays revisited in a human renal proximal tubule cell line. Mol Pharm. 2016 doi: 10.1021/acs.molpharmaceut.5b00821
  29. Nieskens TT et al. A Human Renal Proximal Tubule Cell Line with Stable Organic Anion Transporter 1 and 3 Expression Predictive for Antiviral-Induced Toxicity. AAPS J. 2016 doi: 10.1208/s12248-016-9871-8
  30. van Swelm RP et al. Renal handling of circulating and renal-synthesized hepcidin and its protective effects against hemoglobin-mediated kidney injury. J Am Soc Nephrol.  2016 doi: 10.1681/ASN.2015040461
  31. Gozalpour E. et al. Heterogeneous transport of digitalis-like compounds by P-glycoprotein in vesicular and cellular assays. Toxicol In Vitro 2016 doi: 10.1016/j.tiv.2015.12.009
  32. Jansen J. et al. Human proximal tubule epithelial cells cultured on hollow fibers: living membranes that actively transport organic cations. Sci Rep. 2015 doi: 10.1038/srep16702
  33. Peters E et al. Alkaline phosphatase protects against renal inflammation through dephosphorylation of lipopolysaccharide and adenosine triphosphate. Br J Pharmacol. 2015 doi: 10.1111/bph.13261
  34. Mutsaers HAM et al. Proximal tubular efflux transporters involved in renal excretion of p-cresyl sulfate and p-cresyl glucuronide: Implications for chronic kidney disease pathophysiology. Toxicol In Vitro. 2015 doi: 10.1016/j.tiv.2015.07.020
  35. Schophuizen CM et al. Uremic Toxins Induce ET-1 Release by Human Proximal Tubule Cells, which Regulates Organic Cation Uptake Time-Dependently. Cells. 2015 doi: 10.1016/j.actbio.2014.12.002
  36. Di Mise A et al. Conditionally Immortalized Human Proximal-Tubular Epithelial Cells isolated from the urine of a healthy subject express functional Calcium Sensing Receptor. Am J Physiol Renal Physiol. 2015. doi: 10.1152/ajprenal.00352.2014
  37. Ma L et al. Localization of APOL1 protein and mRNA in the human kidney: nondiseased tissue, primary cells, and immortalized cell lines. J Am Soc Nephrol. 2015 doi: 10.1681/ASN.2013091017
  38. Ivanova EA et al. Endo-Lysosomal Dysfunction in Human Proximal Tubular Epithelial Cells Deficient for Lysosomal Cystine Transporter Cystinosin. PLoS One. 2015 doi: 10.1371/journal.pone.0120998
  39. Schophuizen CM et al. Development of a living membrane comprising a functional human renal proximal tubule cell monolayer on polyethersulfone polymeric membrane. Acta Biomater. 2015 doi: 10.1016/j.actbio.2014
  40. Jansen J et al. A morphological and functional comparison of proximal tubule cell lines established from human urine and kidney tissue. Exp Cell Res. 2014 doi: 10.1016/j.yexcr.2014.02.011
  41. Mekahli D et al. Polycystin-1 but not polycystin-2 deficiency causes upregulation of the mTOR pathway and can be synergistically targeted with rapamycin and metformin. Pflugers Arch. 2014 doi: 10.1007/s00424-013-1394-x.
  42. Mutsaers HA et al. Optimized metabolomic approach to identify uremic solutes in plasma of stage 3-4 chronic kidney disease patients. PLoS One  2013 doi: 10.1371/journal.pone.0071199
  43. Hosper NA et al. Epithelial-to-mesenchymal transition in fibrosis: Collagen type I expression is highly upregulated after EMT, but does not contribute to collagen deposition. Exp Cell Res 2013 doi: 10.1016/j.yexcr.2013.07.014
  44. Schophuizen CMS et al. Cationic uremic toxins affect renal proximal tubule cell functioning through interaction with the organic cation transporter (hOCT2). Pflügers Archives 2013 doi: 10.1007/s00424-013-1307-z
  45. Gorvin CM, et al. Receptor-mediated endocytosis and endosomal acidification is impaired in proximal tubule epithelial cells of Dent's disease patients. Proc Natl Acad Sci2013 doi: 10.1073/pnas.1302063110
  46. Moghadasali R, et al. Mesenchymal stem cell-conditioned medium accelerates regeneration of human renal proximal tubule epithelial cells after gentamicin toxicity. Exp Toxicol Pathol 2013 doi: 10.1016/j.etp.2012.06.002.
  47. Mutsaers H et al. Uremic toxins inhibit renal metabolic capacity through interference with glucuronidation and mitochondrial respiration. Biochim Biophys Acta 2013 doi: 10.1016/j.bbadis.2012.09.006
  48. Iglesias DM et al. Stem cell microvesicles transfer cystinosin to human cystinotic cells and reduce cystine accumulation in vitro. PLoS One. 2012 doi: 10.1371/journal.pone.0042840
  49. Mekahli D et al. Polycystin-1 and polycystin-2 are both required to amplify inositol-trisphosphate-induced Ca2+ release. Cell Calcium. 2012 doi: 10.1016/j.ceca.2012.03.002
  50. Vicinanza M et al., OCRL controls trafficking through early endosomes via PtdIns4,5P(2)-dependent regulation of endosomal actin. EMBO J. 2011 doi: 10.1038/emboj.2011.354
  51. Wilmer MJ et al., Cysteamine restores glutathione redox status in cultured cystinotic proximal tubular epithelial cells. Biochim Biophys Acta 2011 doi: 10.1016/j.bbadis.2011.02.010
  52. Wilmer MJ et al., Novel conditionally immortalized human proximal tubule cell line expressing functional influx and efflux transporters. Cell Tissue Res 2010 doi: 10.1007/s00441-009-0882-y